Ngn2 binding site. .

Ngn2 binding site. Members of the Wnt signaling pathway are predicted as targets of Ngn2 in the cerebral cortex. In contrast, factor-specific binding in closed chromatin involves NGN2 and MyoD1 acting as pioneer-factors, influenced by multi-motifs, rotational spacing, flanking sequences, and specific interaction partners, accounting for subsequent lineage divergence. In contrast, factor-specific binding in closed chromatin involves NGN2 and MyoD1 acting as pioneer factors, influenced by motif variant frequencies, motif spacing, and interaction partners, which together account for subsequent lineage divergence. Oct 10, 2024 · The inclusion of SOX11 did not greatly alter this consensus NGN2-binding motif, which occupied about 84% of all NGN2 targets in U251 cells expressing NGN2 and SOX11. Shared binding sites reside in open chromatin, locally influenced by nucleosomes. Here, we present a comprehensive analysis using sequencing to dissect the molecular mechanisms of how chromatin environments and cofactors restrict NGN2. In practice, mutation of Cdk phosphorylation sites substantially enhances the ability of Ngn2 to drive neuronal differentiation in the cells in which it is expressed, both in vitro and in vivo (Ali et al. Aug 7, 2025 · If genomic binding of NGN2/MyoD1 depend on motif occurrence and DNA accessibility, then differential binding should occur in genomic sites that are initially closed but have higher frequency of NGN2’s and MyoD1’s individually preferred motifs. . , 2011). Generation of defined neuronal subtypes from human pluripotent stem cells remains a challenge. Mar 31, 2008 · Members of the Wnt signaling pathway are predicted as targets of Ngn2 in the cerebral cortex. Aug 7, 2025 · Shared binding sites reside in open chromatin, locally influenced by nucleosomes. Aug 5, 2024 · Shared binding sites reside in open chromatin, locally influenced by nucleosomes. We annotated predicted NGN2 binding sites based on chromatin accessibility to identify potential NGN2 target genes associated with these peaks and performed GO enrichment (Fig. In this study, we present a standardized approach for generating neurons utilizing clonal, targeted-engineered iPSC lines with defined reagents. Upon deletion of Yy1, activation of neuronal enhancers, genes and Sep 9, 2024 · NGN2-driven induced pluripotent stem cell (iPSC)-to-neuron conversion is a popular method for human neurological disease modeling. 4, E to F). We then took advantage of the identification of putative downstream effectors of proneural genes to identify conserved binding sites for Mash1 and Ngn2, then scanned the sequence surrounding them for other conserved TFBSs. Jul 2, 2024 · Instead, we identified Yy1, a transcriptional co-factor recruited by direct interaction with Ngn2 to its target sites. We show that this is achieved by multi-site phosphorylation of Ngn2, which is quantitatively sensitive to cdk levels and quantitatively regulates the stability of Ngn2 binding to promoters of key downstream transcriptional targets, resulting in the regulation of neuronal differentiation. uhwmt md4 pdcr 5t wm8sn xvub11o lob 00cojns 1fhq 4jg1x